现测定蛋白质含量方法有很多:
① 根据物理性质:紫外分光光度法
② 根据化学性质:凯氏定氮法、双缩脲法、Folin-酚试剂法(Lowry法)、BCA法、胶体金法。
③ 根据染色性质:考马斯亮蓝染色法、银染法。
④ 根据其他性质:荧光法。
蛋白定量的测试方法有很多种,其中较为常见的有五种,分别是Bradford法、Bradford斑点试验、Coomassie 斑点试验、紫外分光度检测法及BCA法这五种。
PH0325 Bradford蛋白浓度测定试剂盒 500T/1000T
PH0326 BCA蛋白浓度测定试剂盒(BCA Protein Assay Kit) 250T / 500T
PH0328 Easy-Lowry蛋白定量试剂盒 (Easy-Lowry Protein Assay Kit) 500T/1000T
PH0357 考马斯亮蓝快速染色液(Commassie Blue Fast Staining Solution) 100ml/500ml
PH1399 双缩脲蛋白定量试剂盒(Biuret Protein Quantification Kit) 500T/1000T
PH1400 蛋白快速银染试剂盒(Protein Fast Silver Stain Kit) 20T
PH1399 双缩脲蛋白定量试剂盒(Biuret Protein Quantification Kit) 500T/1000T
PH1400 蛋白快速银染试剂盒(Protein Fast Silver Stain Kit) 20T
PH0311 细菌细胞总蛋白提取试剂盒 (Bacterial Cell Protein Extraction Kit) 50T
PH0313 酵母细胞总蛋白提取试剂盒 (Yeast Proein Extraction Kit) 25T
PH0312 哺乳动物蛋白抽提试剂盒 (Mammalian Protein Extraction Kit) 50T
PH0314 组织蛋白抽提试剂盒 (Tissue Protein Extraction Kit) 25T
PH0315 植物蛋白提取试剂盒 (Plant Protein Extraction Kit) 25T/100T
PH0316 RIPA裂解液(强) RIPA Lysis Buffer (Strong) 100ml
PH0317 RIPA裂解液(中) RIPA Lysis Buffer (Medium) 100ml
PH0318 RIPA裂解液(弱) RIPA Lysis Buffer (Mild) 100ml
PH0319 SDS裂解液 (SDS Lysis Buffer) 100ml
PH0320 蛋白酶抑制剂混合物 (Protease Inhibitor Cocktail) 1ml
PH0321 蛋白磷酸酶抑制剂混合物(100×) Phosphatase Inhibitor Cocktail(100×) 1ml
PH0322 线粒体提取试剂盒 (Mitochondrial Extraction Kit) 50T/100T
PH0324 细胞核蛋白与细胞浆蛋白抽提试剂盒
(Nuclear protein and plasma protein extraction kit) 50T/200T
PH0710 膜蛋白和胞浆蛋白提取试剂盒
Membrane and Cytosol Protein Extraction Kit 50T/100T
PH0352 曲拉通 (Triton X-100) 100ml
PH0350 PMSF (100mM) 1.5ml/10ml
PH1421 NP-40裂解液 / NP-40 Lysis Buffer 100ml
PH0329 BSA标准品 (5mg/ml) 1ml
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